X-Message-Number: 11563
Date: Fri, 16 Apr 1999 09:07:31 -0700 (PDT)
From: Doug Skrecky <>
Subject: ethylene glycol and dry ice storage

Authors
  Sakurada M.  Tsuzuki Y.  Morgavi DP.  Tomita Y.  Onodera R.
Institution
  Animal Science Division, Faculty of Agriculture, Miyazaki University, Japan.
Title
  Simple method for
  cryopreservation of an anaerobic rumen fungus using ethylene
  glycol and rumen fluid.
Source
  FEMS Microbiology Letters.  127(3):171-4, 1995 Apr 1.
Abstract
  The cryopreservation of an anaerobic rumen fungus, Piromyces
  communis OTS1, was examined at -84 degrees C using dimethyl sulfoxide,
  propylene glycol or ethylene glycol as cryoprotectants. Ethylene glycol was
  the most effective agent, combining high survival and low toxicity, followed
  by dimethyl sulfoxide and propylene glycol. Cell-free rumen fluid in the
  cryopreservation medium decreased the toxicity of the
  cryoprotectant agents and also had a protective action per se. A survival of
  80% after 1 year storage was obtained when samples with an initial zoospore
  density of 5 x 10(4) zoospores/ml were equilibrated for 15
  min in medium containing 0.64 M ethylene glycol and 5% cell-free rumen fluid,
  then frozen with dry ice and stored at -84 degrees C.

Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=11563