X-Message-Number: 11571
Date: Sun, 18 Apr 1999 07:56:51 -0700 (PDT)
From: Doug Skrecky <>
Subject: sodium benzoate reduces glycerol toxicity

Authors
  Shah SV.  Walker PD.
Institution
  Department of Medicine (Nephrology), Tulane University School of Medicine,
  New Orleans, Louisiana 70112.
Title
  Evidence suggesting a role
  for hydroxyl radical in glycerol-induced acute renal failure.
Source
  American Journal of Physiology.  255(3 Pt 2):F438-43, 1988 Sep.
Abstract
  Reactive oxygen metabolites, in particular hydroxyl radical, have been shown
  to be important mediators of tissue injury in several models of acute renal
  failure. The aim of the present study was to examine the
  role of hydroxyl radical in glycerol-induced acute renal
  failure, a model for myoglobinuric renal injury. Rats injected with glycerol
  alone (8 mg/kg im following dehydration for 24 h) developed significant renal
  failure compared with dehydrated controls. Rats treated with glycerol and a
  hydroxyl radical scavenger, dimethylthiourea (DMTU), had significantly lower
  blood urea nitrogen (BUN) and creatinine. In contrast, urea, which is
  chemically similar to DMTU but is not a hydroxyl radical scavenger, provided
  no protection. In addition, DMTU prevented the glycerol-induced rise in renal
  cortical malondialdehyde content (a measure of lipid peroxidation that serves
  as a marker of free radical-mediated tissue injury). A second hydroxyl
  radical scavenger, sodium benzoate, had a similar protective effect on renal
  function (as measured by both BUN and creatinine). Because the generation of
  hydroxyl radical in biological systems requires the presence of a trace metal
  such as iron, we also examined the effect of the iron chelator, deferoxamine
  on glycerol-induced renal failure. Deferoxamine was also protective. The
  interventional agents were also associated with a marked reduction in
  histological evidence of renal damage. The protective
  effects of two hydroxyl radical scavengers as well as an iron chelator
  implicate a role for hydroxyl radical in glycerol-induced
  acute renal failure.

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