X-Message-Number: 11586 Date: Tue, 20 Apr 1999 23:25:42 -0700 (PDT) From: Doug Skrecky <> Subject: cysteine and heart preservation Authors Holdefer MM. Wicomb WN. Levy JV. Collins GM. Institution California Pacific Medical Center Research Institute, San Francisco 94115. Title Cardiotonic effects of reduced sulfhydryl amines after preservation of rabbit hearts. Source Journal of Heart & Lung Transplantation. 13(1 Pt 1):157-9, 1994 Jan-Feb. Abstract This investigation was designed to examine the role of glutathione and other reduced sulfhydryl amines during reperfusion of the ischemic rabbit heart. To identify the biochemical features of reduced sulfhydryl amines contributing toward improved myocardial function, we investigated several chemical agents sharing a common property with glutathione (L-leucine, L-glycine, ascorbate, oxidized glutathione, L-cysteine). After a period of 24-hour hypothermic storage of the rabbit heart in a modified University of Wisconsin solution containing polyethylene glycol, the hearts were functionally evaluated on a Langendorff working heart model. The agents were then injected as a bolus (60 mumol/L) during reperfusion, and coronary flow and aortic output were measured. Control hearts were untreated. Reduced sulfhydryl amines (glutathione, L-cysteine) significantly improved coronary flow (p < 0.005) and cardiac output (p < 0.005). Ascorbate, L-leucine, L-glycine, and oxidized glutathione all failed to influence cardiac function. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=11586