X-Message-Number: 11636
Date: Thu, 29 Apr 1999 05:41:01 -0700 (PDT)
From: Doug Skrecky <>
Subject: microwaves better than formaldehyde

Authors
  van Dorp R.  Boon ME.  Kok PG.  Marani E.
Institution
  Leiden Cytology and Pathology Laboratory, The Netherlands.
Title
  Combining microwave
  stabilization and microwave-stimulated
  fixation of brain tissue with microwave-stimulated staining.
Source
  European Journal of Morphology.  33(2):164-73, 1995 Apr.
Abstract
  Microwave stabilization of rat brain tissue
  using saline was compared with microwave-stimulated fixation
  using either formaldehyde or Kryofix. Sections were stained according to the
  microwave-adapted Nissl, Kluver-Barrera, and Bodian methods.
  Depending on the preparative choice the microwave staining
  method had to be adjusted. Optimal combinations of preparative techniques and
  staining methods are feasible. In most cases the microwave
  saline-stabilized and microwave Kryofix-treated sections
  were better suited for application in neurohistology than the
  microwave formaldehyde-treated sections for light
  microscopy.

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