X-Message-Number: 11867 Date: Tue, 1 Jun 1999 08:58:06 -0700 (PDT) From: Doug Skrecky <> Subject: glycerol and DMSO Quote from (J Histochemistry and Cytochemistry 34(10): 1301-1315 1986): "In the present study, initial trials indicated that glycerol was the most promising cryoprotectant and that it did not interfere with any of our standard neuroanatomical or neurohistochemical procedures. However its rate of penetration was relatively slow, requiring very long and potentially deleterious infiltration times where demonstration of histochemical activity was of concern. As a consequence, the 2% DMSO was added to the glycerol solutions not as a cryoprotectant but because it facilitated penetration of the glycerol and reduced total infiltration times by 50%." The above quote was with regard to chemically fixed blocks of monkey brain tissue, being inflitrated with 20% glycerol solutions. IMHO, adding 2% DMSO to standard glycerol solutions now in use with cryonics concerns, would likely be beneficial. Has anyone done any experiments looking into this? Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=11867