X-Message-Number: 12025 Date: Sun, 27 Jun 1999 23:28:23 -0700 (PDT) From: Doug Skrecky <> Subject: much freezing damage occurs during rewarming Authors Fowler AJ. Toner M. Institution Mechanical Engineering Department, University of Massachusetts, Dartmouth 02747, USA. Title Prevention of hemolysis in rapidly frozen erythrocytes by using a laser pulse. Source Annals of the New York Academy of Sciences. 858:245-52, 1998 Sep 11. Abstract This paper reports on the successful recovery of rapidly frozen unprotected erythrocytes by vitrification of the intracellular solution with a laser pulse prior to thawing. Erythrocytes that were frozen at 10,000 degrees C/min exhibited 100% hemolysis when thawed unless they were first irradiated by a 7 ns. laser pulse that selectively targeted the intracellular ice so that it was melted and resolidified into a glass phase. Up to 80% of the cells treated in this way remained intact after thawing. Wright's staining confirmed a healthy cell morphology and the retention of hemoglobin in the laser treated cells. While it is well known that small amounts of intracellular ice can be tolerated by cells, the findings of this study are the first to indicate that intracellular ice may be innocuous even when formed in substantial quantities provided that crystal growth and coalescence can somehow be avoided during warming. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=12025