X-Message-Number: 12698
Date: Tue, 2 Nov 1999 20:20:11 -0800 (PST)
From: Doug Skrecky <>
Subject: inert dehydration with ethylene glycol

Citations: 1-4
<1>
Authors
  Stirling JW.  Coleman M.  Brennan J.
Institution
  Department of Pathology, Flinders Medical Centre, Bedford Park, South
  Australia.
Title
  The use of inert
  dehydration and glycol methacrylate embedding for immunogold
  localization of glomerular basement membrane components.
Source
  Laboratory Investigation.  62(5):655-63, 1990 May.
Abstract
  A protocol is described for the preparation of human
  pathology specimens without fixation, in order to perform immunocytochemistry
  at an ultrastructural level. Using the technique in
  conjunction with immunogold labeling, the basal lamina
  components type IV collagen, laminin, and heparan sulfate have been
  demonstrated in glomerular capillary loops in stored frozen human renal
  tissue. Tissue was thawed and immediately dehydrated with
  the inert cryoprotectant ethanediol
  (inert dehydration) followed by embedding
  in low-acid glycol methacrylate polymerized using the
  accelerator n,n-dimethylaniline. Tissue processed in this way retained
  superior antigenic activity when compared with tissue reprocessed from wax
  blocks and embedded in low-acid glycol methacrylate. Inert
  dehydration is a technique useful for
  the localization of processing sensitive epitopes in routine
  fresh or frozen archival pathologic material. Furthermore,
  high probe densities can be achieved without recourse to etching or enzyme
  treatments.

<2>
Authors
  Sedar AW.
Title
  Electron microscopic demonstration of polysaccharides associated with
  acid-secreting cells of the stomach after
  "inert dehydration".
Source
  Journal of Ultrastructure Research.  28(1):112-24, 1969 Jul.

<3>
Authors
  Koscianek-Malczewska H.  Komender J.  Ostrowski K.
Title
  Investigation of the N and P losses in different media
  used for inert
  dehydration.
Source
  Experientia.  23(4):279-80, 1967 Apr 15.

<4>
Authors
  Pease DC.
Title
  Inert dehydration. A new physical method of
  tissue preparation.
Source
  Medical & Biological Illustration.  16(2):89-97, 1966 Apr.

Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=12698