X-Message-Number: 13065 Date: Thu, 6 Jan 2000 05:09:02 -0800 (PST) From: Doug Skrecky <> Subject: improved fixation Authors De Jong JP. Voerman JS. Leenen PJ. Van der Sluijs-Gelling AJ. Ploemacher RE. Institution Department of Cell Biology, Erasmus University, Rotterdam, The Netherlands. Title Improved fixation of frozen lympho-haemopoietic tissue sections with hexazotized pararosaniline. Source Histochemical Journal. 23(9):392-401, 1991 Sep. Abstract In this study, a simple single-step fixation method for frozen tissue sections is introduced using the hexazotized salt of Pararosaniline as preservative agent. Tissue preservation by this method was shown to be superior to the commonly-used fixation with acetone. Fixation with hexazotized Pararosaniline caused a minimal loss of antigenicity as demonstrated using twenty-three monoclonal antibodies directed against lympho-haemopoietic and stromal cells. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=13065