X-Message-Number: 13065
Date: Thu, 6 Jan 2000 05:09:02 -0800 (PST)
From: Doug Skrecky <>
Subject: improved fixation

Authors
  De Jong JP.  Voerman JS.  Leenen PJ.  Van der Sluijs-Gelling AJ.  Ploemacher
  RE.
Institution
  Department of Cell Biology, Erasmus University, Rotterdam, The Netherlands.
Title
  Improved fixation of
  frozen lympho-haemopoietic tissue sections with hexazotized
  pararosaniline.
Source
  Histochemical Journal.  23(9):392-401, 1991 Sep.
Abstract
  In this study, a simple single-step fixation method for
  frozen tissue sections is introduced using the hexazotized
  salt of Pararosaniline as preservative agent. Tissue preservation by this
  method was shown to be superior to the commonly-used
  fixation with acetone. Fixation with
  hexazotized Pararosaniline caused a minimal loss of antigenicity as
  demonstrated using twenty-three monoclonal antibodies directed against
  lympho-haemopoietic and stromal cells.

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