X-Message-Number: 14074 From: "Shane Brubaker" <> Subject: Question on cyronics procedures Date: Tue, 11 Jul 2000 16:15:28 PDT Hello. I'm interested in cryonics and I was wondering if I could ask some questions about the procedures used. Specifically, is there any fixative (such as glutaraldehyde) used in the perfusion solution. It is my understanding that fixation is necessary to preserve synapses. When freezing a rat brain for instance using either paraformaldehyde or a cryoprotectent solution alone, such as sucrose, the synapses dissolve and are not present when observed via electron microscopy -- only the cell bodies and major trunks of the dendrites are preserved. Although this might make a traditional resuscitation more difficult, it would be more certain to preserve brain structure. I am more concerned with the preservation, for instance for use in a mind uploading procedure, than I am with having my original body resuscitated. Is this done by anyone, and if not could I make a specific request to have it performed on me if I were to arrange suspension through one of the existing organizations? I'd greatly appreciate any information you can give me. Any references in regard to the fixation procedures and level of brain preservation would also be welcome. Thank You, Shane Brubaker Email: Web Page: http://www.geocities.com/Area51/Rampart/7937/index.html Online Resume: http://www.angelfire.com/or/shanebrubaker/resume.html ________________________________________________________________________ Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=14074