X-Message-Number: 14642
From: Keith Rene Dugue <>
Subject: Liposomes?
Date: Sun, 8 Oct 2000 21:07:43 -0700

Yvan Bozzonetti Wrote:

>Now, the question of the day:
>It seems, many good cryoprotectants are very viscous. Has anybody tried to 
>load them in coacervats (sorry, this is the French name, I don't know the
>English translation). They are small vesicles similar to cell wall and
>exploited in some health care products.

>Not only this would permit to use very viscous products, but they can be
>tagged with antibodies, so that they fuse with a given cell population. 
The
>cargo is delivered inside the cell, so that the requested amount of
>cryoprotectant in the general blood system is reduced (less toxicity). the 
>cryoprotectant inside the coacervats may be different of the one in the
>general solution, so one product may be used inside the cells and another
>outside.



Yvan,

Thank you for interjecting a bit of current research into the discussions 
here on Cryonet!
I believe that you are referring to liposomes. Maybe Your suggestion has 
some merit for some CPAs ,however; alkoxylated compounds have been 
developed by 21 CM that have reduced viscosity and good permeation 
kinetics. Greg Fahy and Brian Wowk could address this subject much better 
than I ,but I don't think that the use of liposomes would improve the CPAs 
that have been developed to date. Furthermore, the liposomes must be 
tailored to the chemical being delivered due to the interaction of the 
phospholipids and the CPA. I am not sure how well liposomes function at low 
temperatures. Anyway, some chemicals like alcohols affect liposome 
stability.

I believe the major task at hand is to prove to some degree that memory 
systems can be preserved at cryogenic temperatures within the research 
budget available.


Keith R. Dugue

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