X-Message-Number: 14642 From: Keith Rene Dugue <> Subject: Liposomes? Date: Sun, 8 Oct 2000 21:07:43 -0700 Yvan Bozzonetti Wrote: >Now, the question of the day: >It seems, many good cryoprotectants are very viscous. Has anybody tried to >load them in coacervats (sorry, this is the French name, I don't know the >English translation). They are small vesicles similar to cell wall and >exploited in some health care products. >Not only this would permit to use very viscous products, but they can be >tagged with antibodies, so that they fuse with a given cell population. The >cargo is delivered inside the cell, so that the requested amount of >cryoprotectant in the general blood system is reduced (less toxicity). the >cryoprotectant inside the coacervats may be different of the one in the >general solution, so one product may be used inside the cells and another >outside. Yvan, Thank you for interjecting a bit of current research into the discussions here on Cryonet! I believe that you are referring to liposomes. Maybe Your suggestion has some merit for some CPAs ,however; alkoxylated compounds have been developed by 21 CM that have reduced viscosity and good permeation kinetics. Greg Fahy and Brian Wowk could address this subject much better than I ,but I don't think that the use of liposomes would improve the CPAs that have been developed to date. Furthermore, the liposomes must be tailored to the chemical being delivered due to the interaction of the phospholipids and the CPA. I am not sure how well liposomes function at low temperatures. Anyway, some chemicals like alcohols affect liposome stability. I believe the major task at hand is to prove to some degree that memory systems can be preserved at cryogenic temperatures within the research budget available. Keith R. Dugue NO SPAM -----BEGIN PGP PUBLIC KEY BLOCK----- Version: PGPfreeware 6.5.8 for non-commercial use <http://www.pgp.com> Comment: If you decrypted the above message then store it encrypted mQGiBDULe+YRBADUywd6brECHKAiWuc2uO/fqmnkjTbuawuieSIsYSiW8u2ZEhpH zauVhfn8+qUj0qEDzUsAy6P62zRyxMuyr6XPkqC2IgNYnJU9t0V5erATzV1DipM3 RVJY8TwaByYke2Ze7cALAXR0LcSpWfFDzCUis12WmrxXwsJKaKdo+ow2ywCg/3V8 vG6/7edhuWQaWoLkPE8bFk8D/jsRnV+RMlrz2aClkPKGzhEJ0LMRSrIE0VLATsbd VYNnxi2U/RU7oDiZif/86l4VOs7U1GxcIfPFJpEVMjCRknq4GXiUk4mdG5lWLt3V FikRFAkWePeSMjuAfLuXgxo+TTXvc23C7blKQCejI6rtuuEJL4EFpMYIHDk5FB1f V6b1A/4l1ecDQVgXeEpuQScejADXDdFlAi3JId9W1oGCtJiZuIvetPO9ZgGz17oO MJv5rdyLlP3JlEiTonGYGZHimVPqbAKooJ95ZOmat3IYJ3M6ANWod0WZd0h7ZFiV u/e/dHKw9DY6tza2viL3gugB26VI7JWCMsM0PGd/ONJ/adHNc7QuS2VpdGggUmVu ZSBEdWd1ZSA8a2VpdGguZHVndWVAaW50ZXJuZXRtY2kuY29tPokASwQQEQIACwUC NQt75gQLAwIBAAoJEE79fUZZPbOixEwAoK+KDiymvtybRIhkg4RyNGCmt56EAJ99 91A2zbKAuz9y/UUcxt2PBSo56rkCDQQ1C3vnEAgA9kJXtwh/CBdyorrWqULzBej5 UxE5T7bxbrlLOCDaAadWoxTpj0BV89AHxstDqZSt90xkhkn4DIO9ZekX1KHTUPj1 WV/cdlJPPT2N286Z4VeSWc39uK50T8X8dryDxUcwYc58yWb/Ffm7/ZFexwGq01ue jaClcjrUGvC/RgBYK+X0iP1YTknbzSC0neSRBzZrM2w4DUUdD3yIsxx8Wy2O9vPJ I8BD8KVbGI2Ou1WMuF040zT9fBdXQ6MdGGzeMyEstSr/POGxKUAYEY18hKcKctaG xAMZyAcpesqVDNmWn6vQClCbAkbTCD1mpF1Bn5x8vYlLIhkmuquiXsNV6TILOwAC Agf/aykzIA0XF0OAzcibwNRYL3SgXrZHlyX1V3yjHYOv5pudtNxtUopCj63SkDFD 4EheL7j/EGngu2KbcbbE6plRK2WmdOK4KIuzBgOABoEpglBI6gScwvBDkHezVqyM MdSbe/bzrHFp6G+hKz3fQMuM1VK2PVSYcA+7R7DtIPNh5MIOsmVcqqZsNbh93QlD b2OKvhqitcJqNt8AHQjhQTVI5c5wmzqRtj3xnGrkeBovGfYEXEHJDH6mlQ6aIalL 3z4tDEAeCMlFYxXplU8JQ7xJPhdPgI1zQR1rihJNNBiGrfAAxT8KeS6bcxAo72+5 PXXIXg+tJDsOV6Ihi/ylWt/ygIkARgQYEQIABgUCNQt75wAKCRBO/X1GWT2zom3s AKDssc1uowUMhZObPiKpRNvs9PjUNQCg838E1lzbDC0MGxGSVal5e2LO3Ro= =NGwr -----END PGP PUBLIC KEY BLOCK----- Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=14642