X-Message-Number: 14903 From: Date: Mon, 13 Nov 2000 05:02:09 EST Subject: Re: Frogs and fish Dans un courrier dat du 09/11/00 11:01:32 Paris, Madrid, a crit : << Subject: Re: Frogs and fish Picked up from Jeff Davis, Nov. 8 message: > Marta Sandberg wrote, referring to natural, antarctic-fish cryoprotectant: > > >The last point is moot. The molecules are too large to be absorbed into > >cells. Unless they can be slimmed down they can't be used as a perfusate > >anyway. > > "Can't be used.." is pretty strong language. Yes, I agree. The solution to large molecule problem is know now for a long time. The diphteric toxin is a two componment system, There is a large protein toxin and a penetrator, able to carry the toxin element inside the cell. The toxin can be removed so there is only the carrer mol cule. It can then pick up any other large molecule we want to put inside a cell. There is no more toxicity and even very big proteins can be injected into cells. Yvan Bozzonetti. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=14903