X-Message-Number: 14918
From: 
Date: Tue, 14 Nov 2000 17:26:40 EST
Subject: large molecules into cell compartments

 >Yes, I agree. The solution to large molecule problem is known now for a 
long 
time.
The diphteric toxin is a two component system, there is a large protein 
toxin and a penetrator, able to carry the toxin element inside the cell. The 
toxin can be removed so there is only the carrier molecule. It can then pick 
up any other large molecule we want to put inside a cell. There is no more 
toxicity and even very big proteins can be injected into cells.

>Yvan Bozzonetti.

  This is correct, but to get anti-freezing proteins into cellular organelles 
requires that they have the correct sequences on their N-terminal ends; the 
sequences for most organelles are known. Presumably antifreeze is necessary 
in the mitochondria, lysosomes, etc., so several different antifreeze 
proteins must be synthesized. No real problem, just a slight increase in 
complexity. --Bill Walker

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