X-Message-Number: 15038
Date: Tue, 28 Nov 2000 03:24:26 -0800 (PST)
From: Doug Skrecky <>
Subject: 32'nd update on fly longevity experiments

     This is the 32'nd update on my fly longevity experiments. In Run 
 #31 I retest coconut juice, and synephrine, which improved longevity
 back in Run #28. For comparison purposes, I have reproduced part of 
 Run #28 below as well.


 Run #31                   Percent Survival on Day
 supplement           4  9 12 19 25 29 33 40 49 53 56 60 63 68
 _____________________________________________________________
 control             81 33 22 22 22 15  7  7  0  -  -  -  -  -
 coconut juice       90 90 70 70 40 40 30 30 20 20 10 10 10 10
 elderberry nectar   90 80 80 80 60 60 30 10  0  -  -  -  -  -
 IP6 250 mg          74 50 34 24 18  8  3  3  0  -  -  -  -  -
  +resveratrol 50 mg 88 50 33 33 21 17  4  4  0  -  -  -  -  -
 malvia verticellata 92 69 58 38 19 19 12  0  -  -  -  -  -  -
 resveratrol 50 mg   92 72 44 40 12 16  4  0  -  -  -  -  -  -
 synephrine 15 mg    71 67 46 42 21 21 17  8  0  -  -  -  -  -


 Run #28                   Percent Survival on Day
 supplement         3  7 11 17 20 24 31 35 41 46 51 56
 _____________________________________________________
 control          100 89 68 64 61 57 36 14 14  7  0  -
 coconut juice     96 79 71 71 71 67 58 50 42 38 21  4
 synephrine 15 mg  95 90 60 60 55 55 40 40 30 25 20 10

     The flies used in Run #31 were derived from a rather old breeding
 bottle, which presumably held a lot of older flies. An increased 
 number of doddering flies might account for the higher than normal 
 short-term mortality in both the control bottle, as well as several
 other bottles in this run.
     This time around synephrine did not increase maximum lifespan, 
 though early survival was enhanced. Judging by its effect on flies,
 synephrine might prove to be a non-toxic antiobesity agent for humans.
     Once again coconut juice was associated with superior overall
 survival, increasing both average and maximum survivals in both runs.
 The active ingrediants in coconut juice are unknown, as is the
 mechanism of their operation.
     I included IP6 (inositol hexaphosphate), and resveratrol to test
 thet iron, and copper dependance respectively of fly longevity. Flies 
 fed 4-24 fly food are known to accumulate iron in tissues. Inclusion
 of iron chelators is known to block this accumulation and increase
 longevity slightly. However I use citric acid as a standard additive
 to block pathogen  growth, and this may also reduce iron availability.
 This may be the reason IP6 failed to offer a benefit in the present
 experiment. Resveratrol also proved to be a disappointment.
     One surpise hit was with Knudsen elderberry nectar juice. The
 ingrediants are as follows: filtered water, juices from whole apple,
 elderberry, bosenberry, plum, and grape. I was not impressed with the
 results with either apple juice (Run #11), prune juice (Runs #22 & 24) 
 or  with grape juice (Runs #11 & #30), although grape may offer a very
 minor benefit. Both elderberry and bosenberry juices remain to be
 investigated. Although average longevity was the best with elderberry
 nectar, no benefit on maximum lifespan appeared in this run.
     In Run #32 I retested both coconut, and Knudsen elderberry nectar
 juices. The results were uniformly good. At a 50% dilution both juices
 increased lifespan. At full strength, coconut outperformed 50% juice up
 until the day 37 census. Full strength elderberry improved survival over
 50% nectar at all time points. I will admit to being impressed with
 elderberry nectar. Further experiments investigating this nectar, as
 well as its components are planned for the future.


 Run #32                       Percent Survival on Day
 supplement               5  9 13 21 30 34 37 41 44 49 52 55 59 62 65 68
 _______________________________________________________________________
 control                 91 70 52 48 21 18  9  6  6  0  -  -  -  -  -  -
 coconut juice 50%       94 88 61 52 39 33 30 30 27 15 12  0  -  -  -  -
 coconut juice 100%      97 97 80 70 57 40 30 23 23 10  7  0  -  -  -  -
 elderberry nectar 50%   92 65 65 58 38 35 19 15 12 12 12  8  4  4  4  4
 elderberry nectar 100% 100 91 78 78 59 50 41 22 22 19 19 19 13 13 13  9

     I tried another freezer experiment in my ongoing quest to discover
 a method for implimenting hopefully patentible reversible
 cryopreservation, getting rich, famous, etc. Pycnogenol was added to
 all bottles, since this earlier had been found to be beneficial. 
 Various amounts of polyethylene glycol 200 were added to see if this 
 could further increase survival. I chose a low molecular weight PEG,
 so it would be absorbed from the food into fly tissues.
     Unfortunately adding PEG 200 to fly food results in some extra 
 fluid being released from the food when it is thawed out after
 freezing. If there were any surviving flies, they were all drowned.
     The moral of this story is that experiments testing the 
 effectiveness of cryoprotectants in inhibiting freeze/thaw damage
 are not feasible with the current setup.

 Freezer Run #4  Percent Survival After
 supplement          0 90 minutes
 ________________________________
 pycnogenol 300 mg 100  4
 + PEG 200 17%     100  0
 + PEG 200 33%      94  0

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