X-Message-Number: 15416 Date: Mon, 22 Jan 2001 05:13:47 -0800 (PST) From: Doug Skrecky <> Subject: CI's 75% glycerol It looks like CI is already using a "vitrification" solution. If tissue is fully equilibriated with 75% glycerol, ice crystal formation could not occur. This equilibriation would take time, so water content is probably higher than 25% with the short periods of perfusion used. However as long as tissue water content is below 40%, rapid freezing could result in vitrification. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=15416