X-Message-Number: 15453
Date: Fri, 26 Jan 2001 00:18:04 -0800 (PST)
From: Doug Skrecky <>
Subject: Vitrification: should PEG be a standard additive?

Title
  Twenty-four-hour ice
  storage of rabbit heart.
Source
  Journal of Heart & Lung Transplantation.  13(5):891-4, 1994 Sep-Oct.
Abstract
  Although cardioplegia is limited to 4 hours of ice
  storage, University of Wisconsin solution has successfully
  extended this period to approximately 12 hours. In this study we have
  substituted polyethylene glycol for hydroxyethyl starch in a simplified
  University of Wisconsin solution (Cardiosol). Rabbit hearts were
  ice stored for 24 hours at 0 degrees C in either University
  of Wisconsin solution or Cardiosol (containing either 5% or 10% polyethylene
  glycol). Fresh control hearts were tested immediately after cardiectomy.
  Function was evaluated in an in vitro working heart model for 1 hour with
  aortic afterload at 100 cm H2O. Total cardiac output or the proportion of
  hearts reaching 100 cm H2O were compared. Hearts stored in University of
  Wisconsin solution for 24 hours functioned at 6% of control levels at 15
  minutes of observation. None reached 100 cm H2O or deteriorated further with
  time (p < 0.05). By contrast, hearts stored in 5% Cardiosol showed
  progressive recovery during the 1-hour observation. Of the 13 hearts, 11
  reached 100 cm H2O with a mean cardiac output of 51% of the control value.
  Increasing the concentration of polyethylene glycol to 10% improved cardiac
  output at all observation times, reaching 80% of control heart performance at
  1 hour (control > 10% > 5% > University of Wisconsin solution [p < 0.05]). We
  concluded that 10% polyethylene glycol significantly improved 24-hour
  ice storage and, hence, viability to a
  functional level that matched our previously reported microperfusion results.

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