X-Message-Number: 16447
Date: Wed, 6 Jun 2001 09:54:28 -0700 (PDT)
From: Doug Skrecky <>
Subject: 20% ethylene glycol/propylene glycol solution as a transport medium?

  The following abstract provides evidence of a surprisingly robust
synergism between ethylene glycol and propylene glycol in preventing cell
lysis during refrigerated storage over a 6 month period.
  I can't help but wonder whether this information could be used to reduce
the toxicity of vitrification solutions....
---------- Forwarded message ----------
  Antifreeze solution improves DNA recovery by preserving the integrity of
  pathogen-infected blood and other tissues.
  Clinical & Diagnostic Laboratory Immunology.  7(6):945-6, 2000 Nov.
  Preserving blood samples for shipping and later DNA extraction has been
  performed by cooling, freezing, drying, freeze-drying, and protease
  treatment, among other methods. Most methods to preserve field samples for
  further DNA extraction do not prevent cellular and DNA damage or are useful
  only in preserving them for short periods. This report introduces a novel
  method for blood and tissue that allows preservation in freezing temperatures
  for a prolonged period of time. The solution reported here (20%
  ethylene glycol-propylene
  glycol) preserves cells and tissues integrity, as judged by
  microscopic analysis, and improves DNA yield and quality.

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