X-Message-Number: 16447 Date: Wed, 6 Jun 2001 09:54:28 -0700 (PDT) From: Doug Skrecky <> Subject: 20% ethylene glycol/propylene glycol solution as a transport medium? The following abstract provides evidence of a surprisingly robust synergism between ethylene glycol and propylene glycol in preventing cell lysis during refrigerated storage over a 6 month period. I can't help but wonder whether this information could be used to reduce the toxicity of vitrification solutions.... ---------- Forwarded message ---------- Title Antifreeze solution improves DNA recovery by preserving the integrity of pathogen-infected blood and other tissues. Source Clinical & Diagnostic Laboratory Immunology. 7(6):945-6, 2000 Nov. Abstract Preserving blood samples for shipping and later DNA extraction has been performed by cooling, freezing, drying, freeze-drying, and protease treatment, among other methods. Most methods to preserve field samples for further DNA extraction do not prevent cellular and DNA damage or are useful only in preserving them for short periods. This report introduces a novel method for blood and tissue that allows preservation in freezing temperatures for a prolonged period of time. The solution reported here (20% ethylene glycol-propylene glycol) preserves cells and tissues integrity, as judged by microscopic analysis, and improves DNA yield and quality. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=16447