X-Message-Number: 16667 Date: Sun, 24 Jun 2001 11:06:10 -0700 (PDT) From: Doug Skrecky <> Subject: glycerol and arginine Title Role of nitric oxide in glycerol-induced acute renal failure in rats. Source Nephrology, Dialysis, Transplantation. 9 Suppl 4:78-81, 1994. Abstract EDRF results from the metabolism of L-arginine. N-omega-nitro-L-arginine is a nitric oxide synthase inhibitor (L-arginine competitive inhibitor). Acute renal failure was induced by i.m glycerol (50%) 5 ml/kg bw. L-arginine: 3 mg/kg bw/min for 60 min before and 60 min after glycerol administration. L-arginine inhibitor (150 micrograms/kg bw/min for 120 min). Cin, Cpah and FENa% were measured immediately or 24 h after glycerol (mean of three periods of 20 min). A second series of similar experiments was done in dehydrated (16 h) rats with a high dose of glycerol (50% solution, 10 ml/kg bw). L-arginine ameliorates the severity of ARF immediately after glycerol administration and enhances the recovery of glycerol-induced ARF. The L-arginine inhibitor resulted in a more severe ARF. Urinary cGMP decreased significantly after glycerol administration. It is concluded that nitric oxide has an important pathogenetic role in the glycerol induced ARF. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=16667