X-Message-Number: 16956
From: 
Date: Wed, 11 Jul 2001 03:19:41 EDT
Subject: LN2 lighting bug / fire fly results

I couged two fire flies this evening with a pillow case.  Each easily 
transferred live and well into his own Nalgene cryogenic test tube (i.e., 
two-inch-long-ampules).  I added the screw on the tops, tied the two ampoules 
together with a "twistem" and a rubberband.  I tied a long string to it with 
a heavy finger ring for a weight near the remaining ample.  Then I lifted the 
lid on the cryostorage unit and submerged them.  After two and a half hours I 
removed the payload, removed one of the ampules and put the other one back in 
LN2.

My prepaired work area was exceptionally well lit.  I unscrewed the ampoule 
and the fly slid right out dead (errr...terminally comatose).  I positioned 
my knife directly on target and then turned out the light.  The fire fly was 
glowing on it's own!  This was quite unexpected.  The light faded over time 
but was still dectable still detectable 1.5 hours later.  

Regards,

David C. Johnson, Raleigh

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