X-Message-Number: 18111
Date: Fri, 7 Dec 2001 09:02:34 -0800 (PST)
From: Doug Skrecky <>
Subject: glycerol/borate combination for vitrification?

Perfuse first with glycerol, then with borate.
Like with trehalose/borate, I would expect the Tg' to be increased.
Perhaps vitrification might occur with a lower concentration of glycerol?

---------- Forwarded message ----------
Title
  Glycerol-enhanced separation of DNA fragments in entangled
  solution capillary electrophoresis.
Source
  Analytical Chemistry.  66(23):4210-4, 1994
  Dec 1.
Abstract
  (Hydroxyalkyl)methylcellulose additives to borate buffers have been used to
  create physically entangled solutions for fractionation of biological
  molecules by high-performance capillary electrophoresis, i.e., ESCE. We have
  observed that addition of glycerol to an entangled solution
  containing (hydroxypropyl)methylcellulose (HPMC) in Tris-borate buffer
  markedly improved the separation of double-strand DNA molecules ranging from
  less than 100 bp to about 1 kb by ESCE. This range of DNA fragment sizes is
  well suited to many PCR-based studies. We attribute the improved ESCE
  performance to the borate present in the buffer system. Borate can form
  dimeric 1:2 borate:didiol complexes with both glycerol and
  HPMC, acting as a central linkage to allow an entangled solution with
  different pore size to form chemically.

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