X-Message-Number: 20816
From: 
Date: Thu, 9 Jan 2003 09:58:03 EST
Subject: perfusate washout

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Yvan Bozzonetti wrote in part:

>Because most of the anti-freeze can't be extracted by simple blood 
circulation, in->situ light break-down is a necessity.

He implied that no present procedure, or any similar procedures, can 
satisfactorily get rid of the cryoprotectant.

This is simply not the case. The work of Fahy et al with rabbit kidneys is 
still not 100% successful, after many years of work, but it is very close, 
since according to reports they have gotten around 50% viability 
clinically--half the specimens allowed long term survival of rabbits using 
previously vitrified kidneys. 50% isn't good enough for organ transplants, 
but it's certainly good enough for proof of principle.

Likewise, Dr. Pichugin's work with brain slices at CI indicates that washout 
of perfusate will not be a major problem, although final proof still lies 
ahead.

Robert Ettinger
Cryonics Institute
Immortalist Society
www.cryonics.org

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