X-Message-Number: 24547
Date: Mon, 23 Aug 2004 05:16:13 -0700 (PDT)
From: Doug Skrecky <>
Subject: 145'th update onfly longevity experiments

(I was told the 145'th update never reached this list.)
---------- Forwarded message ----------
    This is the 145'th update of my fly longevity experiments. Average
temperature was 25.2 C during this run. Estimated maximal longevity using
the formula (363 - T*11.2) is 81 days.
    This run looks at some doses of amino acids that are higher than those
tested before. Control longevity was excellent in this run. This might be
due to the increase in citric acid from 1/8 tsp to 1/2 tsp in all bottles.
Sucrose 1 tsp was added as well.
    Although some amino acids were toxic when added in excess, at least
leucine, and isoleucine appeared to be safe. Arginine looked good during
the first month, though survival subsequently tailed off. This might be
due to the surface wetness induced in the fly food by the added arginine.
At young ages, flies walk mostly on the sides of the milk bottle. As they
age they spend much more time walking on the fly food at the bottom of the
bottles. The wetness of arginine laced food, may have made the aged flies
dirty, and encouraged bacterial infections.

Run #145                     Percent Survival on Day
supplement         7  12  21  26  31 36 41 53 62 68 74 79 84
____________________________________________________________
control one      100 100 100 100 100 97 85 55 15  6  3  0  -
control two      100 100 100  98  98 96 93 64 24 22 20  4  0
alanine  2 tsp   100  85   6   0   -  -  -  -  -  -  -  -  -
arginine 2 tsp   100 100 100 100 100 84 69 19  3  0  -  -  -
cysteine 2 tsp     0   -   -   -   -  -  -  -  -  -  -  -  -
glycine 2 tsp     59  32   0   -   -  -  -  -  -  -  -  -  -
isoleucine 2 tsp 100 100  96  96  96 93 89 57 43 18  4  0  -
leucine 2 tsp    100 100  96  96  96 96 92 52 32 20  4  0  -
taurine 2 tsp     97  90  71  55  55 53 42  8  0  -  -  -  -
threonine 2 tsp   57   0   -   -   -  -  -  -  -  -  -  -  -
valine 2 tsp     100 100 100  93  83 43  3  0  -  -  -  -  -

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