X-Message-Number: 25214 Date: Sat, 4 Dec 2004 12:26:56 -0800 (PST) From: Doug Skrecky <> Subject: 160'th update on fly longevity experiments This is the 160'th update of my fly longevity experiments. Average temperature was 25.5 C during this run. Estimated maximal longevity using the formula (363 - T*11.2) is 78 days. Here I examine the effect of varying levels of moisture, and citric acid. This run further confirms the current harmfulness of citric acid to flies with a low pathogen load. Reducing moisture level also appears to be harmful. It is interesting that both dehydration and acidification can both reduce bacterial growth, yet both of these alterations hurt, rather than help flies. Run #160 Percent Survival on Day supplement 16 21 26 31 36 41 46 51 66 73 83 _____________________________________________________________ no citric +2 tsp water 96 93 93 75 64 61 50 39 0 - - " +1 tsp water 91 91 91 83 70 65 61 44 13 9 0 " (usual water) 94 94 88 88 77 77 71 65 12 0 - " -1 tsp water 79 75 75 71 63 63 63 58 8 0 - " -2 tsp water 89 74 59 56 52 37 22 22 7 0 - citric 1/8 tsp +2 tsp water 81 76 71 67 48 48 48 38 14 5 0 " +1 tsp water 52 44 44 41 33 26 26 19 0 - - " (usual water) 60 60 55 55 55 45 35 30 10 5 0 " -1 tsp water 56 44 33 33 33 22 22 22 6 0 - " -2 tsp water 71 57 57 52 48 29 19 24 14 5 0 citric 1/2 tsp +2 tsp water 41 33 30 26 19 15 15 7 4 4 0 " +1 tsp water 87 57 44 39 35 26 26 17 4 0 - " (usual water) 80 50 40 25 20 15 10 5 0 - - " -1 tsp water 65 48 43 43 38 38 33 30 3 0 - " -2 tsp water 35 24 15 12 9 9 3 0 - - - Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=25214