X-Message-Number: 25358 Date: Sat, 18 Dec 2004 21:16:45 -0800 (PST) From: Doug Skrecky <> Subject: Cardiolipin may be a key regulator of aging Biochem Biophys Res Commun. 2004 Oct 22;323(3):739-42 Cardiolipin induces premature senescence in normal human fibroblasts. Lipids seem to have various roles in cellular senescence. We found that cardiolipin very sensitively inhibits growth of normal human fibroblasts, whereas other phospholipids do not at 100 times higher concentrations. Growth arrested cells showed morphology similar to those of normally senesced cells and strongly induced senescence-associated beta-galactosidase. Senescence markers such as the p21(waf1/sdi-1), fibronectin, and collagenase-I genes were significantly upregulated by cardiolipin. In addition, caldiolipin significantly increased in normally senesced human fibroblasts leaving other phospholipids unaltered. These results suggest that accumulation of cardiolipin is one of the causes for replicative senescence. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=25358