X-Message-Number: 26310
Date: Fri, 10 Jun 2005 23:46:01 -0700 (PDT)
From: Doug Skrecky <>
Subject: aging pigments reversed by cholecystokinin

World J Gastroenterol. 2005 Jan 28;11(4):551-6
Effect of cholecystokinin on experimental neuronal aging.
    AIM: To observe the effect of cholecystokinin (CCK) on
lipofusin value, neuronal dendrite and spine ultrastructure, and
total cellular protein during the process of experimental neuronal
aging. METHODS: Experimental neuronal aging study model was
established by NBA2 cellular serum-free culture method. By using
single intracellular lipofusin value from microspectrophotometry,
morphology of neuronal dendrites and spines from the scanner
electron microscopy, and total cellular protein as the indexes of
experimental neuronal aging, we observed the effect of CCK8 on the
process of experimental neuronal aging. RESULTS: Under the
condition of serum-free culture, intracellular fluorescence value
(%) increased with the extension of culture time (1 d 8.51+/-3.43;
5 d 10.12+/-3.03; 10 d 20.54+/-10.3; 15 d 36.88+/-10.49; (b)P<0.01).
When CCK was added to serum-free culture medium, intracellular
lipofusin value (%) decreased remarkably after consecutive CCK
reaction for 10 and 15 d (control 36.88+/-10.49; 5 d 32.03+/-10.01;
10 d 14.37+/-5.55; 15 d 17.31+/-4.80; (b)P<0.01). As the time of
serum-free culturing was prolonged, the number of neuronal dendrite
and spine cells decreased. The later increased in number when CCK8
was added. CCK8 could improve the total cellular protein in the
process of experimental neuronal aging. CONCLUSION: CCK8 may
prolong the process of experimental neuronal aging by maintaining
the structure and the number of neuronal dendrite and spine cells
and changing the total cellular protein.

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