X-Message-Number: 26345 From: "Basie" <> Subject: For Anthony Date: Thu, 16 Jun 2005 22:11:30 -0400 Step 1. Before an embryo can be frozen, all the water that it contains must be removed. Since water expands in size as it turns to ice, water inside the embryo would burst (kill) the embryo if we simply placed it in the freezer. To prevent the embryo from shriveling as the water is extracted, we replace the water with antifreeze. Antifreeze is a solution that does not expand in size when it freezes. The embryo is cooled to room temperature as the water is replaced with antifreeze. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=26345