X-Message-Number: 29336
From: "Basie" <>
Subject: Observation and identification of metabolites emerging during...
Date: Mon, 19 Mar 2007 17:41:28 -0500

1: Magn Reson Med. 2002 Nov;48(5):915-20.  Links
Observation and identification of metabolites emerging during postmortem 
decomposition of brain tissue by means of in situ 1H-magnetic resonance 
spectroscopy.Ith M, Bigler P, Scheurer E, Kreis R, Hofmann L, Dirnhofer R, 
Boesch C.
Department of Clinical Research (MR Spectroscopy and Methodology), 
University Bern, Switzerland.

Postmortem decomposition of brain tissue was investigated by (1)H-magnetic 
resonance spectroscopy (MRS) in a sheep head model and selected human cases. 
Aiming at the eventual estimation of postmortem intervals in forensic 
medicine, this study focuses on the characterization and identification of 
newly observed metabolites. In situ single-voxel (1)H-MRS at 1.5 T was 
complemented by multidimensional homo- and heteronuclear high-resolution NMR 
spectroscopy of an extract of sheep brain tissue. The inclusion of spectra 
of model solutions in the program LC Model confirmed the assignments in 
situ. The first postmortem phase was characterized mainly by changes in the 
concentrations of metabolites usually observed in vivo and by the appearance 
of previously reported decay products. About 3 days postmortem, new 
metabolites, including free trimethylammonium, propionate, butyrate, and 
iso-butyrate, started to appear in situ. Since the observed metabolites and 
the time course is comparable in sheep and human brain tissue, the model 
system seems to be appropriate. Copyright 2002 Wiley-Liss, Inc.

PMID: 12418008 [PubMed - indexed for MEDLINE]
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