X-Message-Number: 31987
Date: Mon, 21 Sep 2009 01:19:24 +0400
Subject: CAS freezers in Europe
From: Alexei Potapov <>


>Here is a PDF from the Grimsby institute where its mentioned. 
http://ktn.globalwatchonline.com/epicentric_portal/binary/com.epicentric.contentmanagement.servlet.ContentDeliveryServlet/FOP/Public/Events/Slide%2520show%2520of%2520events/CAS%2520presentation-%2520mike%2520dillon.pdf

Thanks Leo for this pdf. So there's at least 2 more of CAS in Europe -
in France and Spain.


>If a CAS freezer don't supercool or vitrifies, but keeps cells undamaged by 
whatever way,  then we will find that out later, the important thing is that it 
(probably) works.

What's most important it can (probably) remove at least a part of ice
damage. And surely it doesn't have modern vitrification solution
nonspecific toxicity effects both during perfusion and thawing.

>Maybe somebody must buy a small CAS freezer and experiment with it.

Guys from Grimsby institute think to do "commercial research" and
they'll got LN2 table top system
so no need to buy them. We need just make a good research plan
(including contacts with japanese researchers) and fundrise some
money.

It may be also very important to bring some theoretical calculation
before testing CAS. Owada dont need of "ideal" freezing of bio sampes
because of it's "food processing" primary goals (like food color or
taste). And i'm pretty sure he doesnt do complete frequency spectrum
and field intencity research. he just no practical need of it.

some parts out of it;
>Liquid nitrogen table top system (June 2009 installation)
>Desktop CAS system for the preservation of harvested stem cells.


>Freeze a Woodfrog or something to liquid Nitrogen  temperatures, and try to 
bring it back to live after that.

i think it will be very interesting to low minimal survival temp of
Woodfrog or other animal with CAS.

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