X-Message-Number: 31993
Date: Tue, 22 Sep 2009 10:05:57 -0700 (PDT)
From: 
Subject: Polyampholytes as low toxic efficient cryoprotective agents w...

Biomaterials. 2009 Sep;30(27):4842-9. Epub 2009 Jun 9.

Polyampholytes as low toxic efficient cryoprotective agents with antifreeze 
protein properties.

    Matsumura K, Hyon SH. Department of Medical Simulation Engineering, 
    Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, 
    Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.

    Dimethyl sulfoxide (DMSO) has been used for several decades as the most 
    efficient cryoprotective agent (CPA) for many types of cells and tissues in 
    spite of its cytotoxicity and its effects on differentiation. Here we report
    that polyampholytes with an appropriate ratio of amino and carboxyl groups 
    show higher cryopreservation efficiency and lower cytotoxicity than DMSO. 
    Culture medium solutions of epsilon-poly-L-lysine (PLL) with more than 50 
    mol% of amino groups carboxylated showed excellent post-thaw survival 
    efficiency of 95% murine L929 cells, and rat mesenchymal stem cells fully 
    retained the potential for differentiation without serum. We also found that
    carboxylated PLLs showed antifreeze protein properties, such as ice 
    recrystallization inhibition, which may contribute to successful 
    cryopreservation by membrane protection. Thus, these polyampholytes can 
    replace DMSO as new materials for CPAs in various preserving functions and 
    will also be useful in studies elucidating the mechanisms of 
    cryopreservation.
PMID: 19515417

Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=31993