X-Message-Number: 32864
Date: Fri, 24 Sep 2010 11:10:03 -0700 (PDT)
From: 
Subject: Electrolyzed-reduced water for cryopreservation?


As far as I am aware, electrolyzed-reduced water has never been tested in either
transplantation, or cryopreservation fluids. Could this reduce cryoprotectant 
toxicity?

Biomed Res. 2009 Oct;30(5):263-9.

Electrolyzed-reduced water inhibits acute ethanol-induced hangovers in 
Sprague-Dawley rats.

Park SK, Qi XF, Song SB, Kim DH, Teng YC, Yoon YS, Kim KY, Li JH, Jin D, Lee KJ.
Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei 
University, Wonju, Gangwon, Republic of Korea.
Abstract

    Ethanol consumption disturbs the balance between the pro- and anti-oxidant 
    systems of the organism, leading to oxidative stress. Electrolyzed-reduced 
    water (ERW) is widely used by people in East Asia for drinking purposes 
    because of its therapeutic properties including scavenging effect of 
    reactive oxygen species. This study was performed to investigate the effect 
    of ERW on acute ethanol-induced hangovers in Sprague-Dawley rats. Alcohol 
    concentration in serum of ERW-treated rats showed significant difference at 
    1 h, 3 h and 5 h respectively as compared with the rats treated with 
    distilled water. Both alcohol dehydrogenase type 1 and acetaldehyde 
    dehydrogenase related with oxidation of alcohol were significantly increased
    in liver tissue while the level of aspartate aminotransferase and alanine 
    aminotransferase in serum was markedly decreased 24 h after pre-oral 
    administration of ERW. Moreover, oral administration of ERW significantly 
    activated non-ezymatic (glutathione) and enzymatic (glutathione peroxidase, 
    glutathione-S-transferase, Cu/Zn-superoxide dismutase and catalase) 
    antioxidants in liver tissues compared with the control group. These results
    suggest that drinking ERW has an effect of alcohol detoxification by 
    antioxidant mechanism and has potentiality for relief of ethanol-induced 
    hangover symptoms.
PMID: 19887722
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http://www.jstage.jst.go.jp/article/biomedres/30/5/263/_pdf

Biol Pharm Bull. 2007 Feb;30(2):234-6.

Preservative effect of electrolyzed reduced water on pancreatic beta-cell mass 
in diabetic db/db mice.

Kim MJ, Jung KH, Uhm YK, Leem KH, Kim HK. Department of Obesity Management, 
Graduate School of Obesity Science, Dongduk Women's University, Seoul, South 
Korea.
Abstract

    Oxidative stress is produced under diabetic conditions and involved in 
    progression of pancreatic beta-cell dysfunction. Both an increase in 
    reactive oxygen free radical species (ROS) and a decrease in the antioxidant
    defense mechanism lead to the increase in oxidative stress in diabetes. 
    Electrolyzed reduced water (ERW) with ROS scavenging ability may have a 
    potential effect on diabetic animals, a model for high oxidative stress. 
    Therefore, the present study examined the possible anti-diabetic effect of 
    ERW in genetically diabetic mouse strain C57BL/6J-db/db (db/db). ERW with 
    ROS scavenging ability reduced the blood glucose concentration, increased 
    blood insulin level, improved glucose tolerance and preserved beta-cell mass
    in db/db mice. The present data suggest that ERW may protects beta-cell 
    damage and would be useful for antidiabetic agent.
PMID: 17268057
Free text>
http://www.jstage.jst.go.jp/article/bpb/30/2/234/_pdf

Biochem Biophys Res Commun. 1997 May 8;234(1):269-74.

Electrolyzed-reduced water scavenges active oxygen species and protects DNA from
oxidative damage.

Shirahata S, Kabayama S, Nakano M, Miura T, Kusumoto K, Gotoh M, Hayashi H, 
Otsubo K, Morisawa S, Katakura Y. Institute of Cellular Regulation Technology, 
Graduate School of Genetic Resources Technology, Kyushu University, Fukuoka, 
Japan.
Abstract

    Active oxygen species or free radicals are considered to cause extensive 
    oxidative damage to biological macromolecules, which brings about a variety 
    of diseases as well as aging. The ideal scavenger for active oxygen should 
    be 'active hydrogen'. 'Active hydrogen' can be produced in reduced water 
    near the cathode during electrolysis of water. Reduced water exhibits high 
    pH, low dissolved oxygen (DO), extremely high dissolved molecular hydrogen 
    (DH), and extremely negative redox potential (RP) values. Strongly 
    electrolyzed-reduced water, as well as ascorbic acid, (+)-catechin and 
    tannic acid, completely scavenged O.-2 produced by the hypoxanthine-xanthine
    oxidase (HX-XOD) system in sodium phosphate buffer (pH 7.0). The superoxide
    dismutase (SOD)-like activity of reduced water is stable at 4 degrees C for
    over a month and was not lost even after neutralization, repeated freezing 
    and melting, deflation with sonication, vigorous mixing, boiling, repeated 
    filtration, or closed autoclaving, but was lost by opened autoclaving or by 
    closed autoclaving in the presence of tungsten trioxide which efficiently 
    adsorbs active atomic hydrogen. Water bubbled with hydrogen gas exhibited 
    low DO, extremely high DH and extremely low RP values, as does reduced 
    water, but it has no SOD-like activity. These results suggest that the 
    SOD-like activity of reduced water is not due to the dissolved molecular 
    hydrogen but due to the dissolved atomic hydrogen (active hydrogen). 
    Although SOD accumulated H2O2 when added to the HX-XOD system, reduced water
    decreased the amount of H2O2 produced by XOD. Reduced water, as well as 
    catalase and ascorbic acid, could directly scavenge H2O2. Reduce water 
    suppresses single-strand breakage of DNA b active oxygen species produced by
    the Cu(II)-catalyzed oxidation of ascorbic acid in a dose-dependent manner,
    suggesting that reduced water can scavenge not only O2.- and H2O2, but also
    1O2 and .OH.
PMID: 9169001

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