X-Message-Number: 3655
Date: 10 Jan 95 15:01:10 EST
From: yvan Bozzonetti <>
Subject: CRYONICS : Colchicum and aspirin. + Nuclear Frankeinstein


	Aspirin is a plant derived durg, it was found with a curious 
reasonning : Arthritis is associated with wet environment and marsh domain, 
so God must have provided the cure in the same location... So aspirin 
(salicilic acid) was discovered in willow bark. (willow = salicine in old 
french). here, I apply the same reasonning: colchicum flower are the first 
to get out of snow in January. that look somewhat as the cover picture of 
The Immortalist for some month now. So I suggest to use some extract of 
these flowers in cryonics perfusate. It could be a sign for getting soon of 
the snow ( the cryonics state ). Unconvinced? Well, then I must invent a 
good story with plenty of pseudoscience. (magy is better).

	I have spoken before about tubulin molecules inside the cell; They 
form rings at the cell's center and microtubules elsewhere; These 
microtubules are continually assembled at cell center and dismantled near 
the outher membrane; the free tubulin molecules get then back to the center 
where they assemble themselve anew. The assembly process is very 
temperature dependent, on the contrairy, peripheral separation runs nearlly 
always at the same speed.

	When a cell is cooled down, its microtubules shorten because they 
are assembled more slowly and disassembled at an unabatted peace. After a 
sufficient time at a sufficiently low temperature, there are no more 
microtubules , only the central rings remain. In this state, a cell can't 
divide: the microtubules are the driving motor behind the chromosome 
separation process and the pinch off of the membrane giving two separate 
cells.

	In a warm environment, the microtubules growth start anew from the 
central rings; now, if the temperature get too low for too long, even the 
central structure disolves and can't start to assemble spontaneously from 
nothing. Often, cells have some pore communication with other cells. If one 
of them has its central structure, it will send some microtubules to the 
deprived ones and from here, all will be build back.

	If there is no such cell in the environment, all the cell group has 
lost its capacity to reproduce. Colchicum flowers contain a poverful poison 
: the colchicine. that product enter the cell and cap the outer end of 
microtubules so that they can't anymore broke into separate componements. 
At the cell center, tubuline molecules are assembled until there is no more 
free molecules. There are then very long tubules in the cell but the 
conveyor belt function stops. There is no more cell division again. On the 
other hand, there are super disolver able to counteract the colchicine 
effect. So this toxic is not really a problem.

	It could be very useful in the cryonics cooling process: it would 
stop the microtubule destruction. Works ar Cryonics Institute have 
demonstrated the value of slow cooling, against the cracking problem. This 
is very harmful for the inner cell microtubule system, so it would be 
interesting to use colchicine to solve the problem.

	-------------------

Now, the message you will not spared:

	Frankeinstein goes nuclear !

	Mary Shelley's Dr. Frankeinstein brougth back to life its creature 
with electric discharges. that was good when electricity was an absolute 
novelty, now I think the technology of nuclear reactor would be more 
appropriate. I don't use precious waveband to produce here a full course on 
nuclear reactors, at least for that time; So I limit myself to the most 
elementary reactor desing where the active elements are simply bathed in 
heavy water (D2O). This is used as a thermal exchanger and a neutron 
slowing medium. In the cryonics thawing process, there is too a thermal 
problem, so why no use heavy water ?

	This product is a devious poison: it looks and tastes as ordinary 
water but kill very effectively ( an idea for a perfect crime). Its action 
is very simple: it dissolve the microtubules system even at room 
temperature. So it mimick the effect of a long, slow cooling. It neutralize 
too the colchicine effect. I think it would be usefull both, at thawing and 
to study the cooling effect on small sample at room temperature.

	Y.B.

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