X-Message-Number: 3926
Newsgroups: sci.cryonics
From:  (Michael Clive Price)
Subject: Re: -79xC and glycerol Molarity
References: <3imv3c$>
Date: Sun, 26 Feb 1995 08:51:52 +0000
Message-ID: <>

My thanks to Brian Wowk and Mike Darwin for their answers about molarity
and cracking.

Does anyone remember offhand what the glycerol concentrations were in
the patients that Alcor took over control of that showed evidence of
cracking?  Is it possible that their cracking was due to too low a
concentration of CPA?  What evidence is there of cracking at high
molarities?  What I wonder is whether Ettinger's no cracking results
(still to be confirmed) are a result of the relativity high CPA
concentrations (5-6M) (in comparison with Suda (2M) and Pegg <=3M))
rather than the cooling/re-warming rates.

I am delighted to hear that Suda did more work, but why oh why
didn't/doesn't he ever publish all the stuff!  (Is he still alive,
Mike?)  BTW I got an email wondering if I really meant that Suda had
stored and revived brains from 7 years storage at -20C -- the
temperature didn't seem cold enough.  Yes, that is what I meant.  What
Suda's experiments showed was that there was a slow deterioation over
this time period (the shorter the storage at -20 C the better the
revival, so this isn't evidence in favour of permafrost burials) and the
brains stored at lower temperatures (-60C and -90C) were markedly more
damaged, presumably (hopefully!) because of the low CPA concentraion
used.

Michael Price                        

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