X-Message-Number: 5352 From: (JensTroll) Newsgroups: sci.cryonics Subject: Re: High Pressure Cryonics Date: 5 Dec 1995 00:48:13 -0500 Message-ID: <4a0met$> References: <> Ok, but, , 1) I suspect that you could form variations on ice-iii at lower pressures using additives in solution with the water. My question is sir does anyone know whether or not physical experiments have been done to investigate the phase change with additives. 2) I strongly suspect that the damage to the tissues can be vary rate dependent, and highly dependent on partial pressures of gases disolved in your working liquid. As in how fast do you apply/ drop the pressure and whether you have any gas bubbles in the system. I have done some experiments along these lines at LSU while a graduate student there. I pressureized mouse embryos to ~ 30,0000 psi. We the dropped the pressure and examined the embryos under microscope. The damage was very extensive, and characteristic of explosive decompression in my opinion and that of some biology grad students I was working with. My technical problem at thetime was that I could not make a mechanism (within my out of pocket budget.) that could deliver or drop those pressures at a slow rate. Can you please tell me whether some work has been done in those areas and where it might be published. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=5352