X-Message-Number: 5836 From: (RAMole) Newsgroups: sci.cryonics Subject: Methods of perfusing Glycerine Date: 26 Feb 1996 03:20:43 -0500 Message-ID: <4grqgr$> I caught the tail end of a TV news item which gave the impression that small toads can be frozen solid and revived because they manage to infuse glycerine around their cells, preventing the formation of large ice crystals. And that rat hearts and maybe larger ones have been frozen and thawed this way, but that it's hard to get the glycerine distributed into all the parts of larger object, or hard to do it before the tissue dies. If this is correct, it seems to boil down to a question of how to make glycerine perfuse through tissue quickly. Has anyone tried simple pressure? For example, filling the circulatory system with it and raising the "blood" pressure to the max that can be withstood? I think there are also experiments with delivery of drugs via transdermal patches. At present, only small molecules will go through the skin this way, but by applying a charge to the patch, they're finding they can force through much larger ones. Could a voltage differential speed the perfusion of glycerine? On a different topic, what's the best textbook on the whole science of cryogenics? Alan Mole Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=5836