X-Message-Number: 6242 Date: Mon, 20 May 1996 23:23:47 -0700 (PDT) From: Doug Skrecky <> Subject: CryoCare Recieves New Patient for Cryopreservation In message 6238 Charles Platt <> wrote: > Henrietta Popper is now making her slow journey down to -196 > Celsius, and Andrew says that his only regret is in failing > to sign her up earlier. > I hope Charles is not implying here that the body of Henrietta Popper is being cooled slowly to -196 C. Experiments have found that in the absence of cryoprotectants slow cooling completely destroys the viability of cells. A procedure of slow cooling to about -10 C to dehydrate cells so that intracellular freezing does not occur, followed by rapid cooling is required for cells to survive. "Nonequilibrium Freezing of One-Cell Mouse Embryos" 1908-1921 Vol.64 June 1993 Biophysical Journal. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=6242