X-Message-Number: 7619 From: Date: Sun, 02 Feb 1997 09:22:29 -0400 (EDT) Subject: Advise for Jack van Loon Date sent: 2-FEB-1997 09:04:47 Most of my experience is with insect cell lines. This I can suggest: You will have to freeze different batches of cells under different conditions until you succeed. There are lines of thought on freezing cells: 1) Immediately in LN. 2) 1hr at -70 C then in LN. The latter does damage some cells because DMSO contains toxic by-products. You probably can store cells at -20 C if you use very pure DMSO (and very expensive). You will find that such DMSO is sold by cell culture reagent providers and not Merck or Sigma. J.C. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=7619