X-Message-Number: 7802
Date: Tue, 4 Mar 1997 05:03:18 -0800 (PST)
From: Doug Skrecky <>
Subject: successful revival of thawed mouse hearts

    Fetal mouse hearts were revived successfully from liquid nitrogen
 storage  back in 1974 by the MRC Transplantation Group at the University
 of Alberta in Edmonton, Canada. Cryopreservation fluid included 10% DMSO
 and 10% FCS (fetal calf serum) in Hepes buffer. Deletion of either of
 these two components eliminated survival. In all 59% of 54 treated hearts
 used showed strong electrical activity after being thawed out.

 Reference: Cryobiology 11: 28-32 1974


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