X-Message-Number: 7807 From: "Jan Coetzee" <> Subject: Cryo Journal Club 4 Date: Tue, 4 Mar 1997 17:32:31 -0500 This is a multi-part message in MIME format. ------=_NextPart_000_01BC28C2.09CFA1A0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 7bit <H3>Critical Cooling and Warming Rates to Avoid Ice Crystallization in Small Pieces of Mammalian Organs Permeated with Cryoprotective Agents</H3> J. F. Peyridieu, A. Baudot, P. Boutron, J. Mazuer, J. Odin, A. Ray, E. Chapelier, E. Payen, J. L. Descotes<P> <P>Measurements were made by differential scanning calorimetry on small pieces of rabbit kidney permeated with 2,3-butanediol containing mainly the levo- and dextro-isomers. The critical cooling rates necessary to vitrify the pieces of organ, and the corresponding critical warming rates which are required to avoid crystallization in the vitrified samples, were determined. The dynamic method used for these determinations is described. The glass-forming tendency and the stability of the amorphous state were both greater in the kidney tissue samples than in the bulk cryoprotective solution. This result is discussed in the context of the lowering of the freezing point of water in emulsions and the promotion of supercooling in hydrogels and porous materials. In corresponding experiments with rat hearts impregnated with 1,2-propanediol, only the critical warming rate was reduced. Cryobiology, v 33, n 4, August 1996. Cryo Journal Club. One can conclude from this experiment that it would be difficult to avoid crystallization during the rewarming of tissue from vitrification. Before one stops other freezing procedures of cryo patients one might want to give serious consideration to this problem. J.C. Your participation will be appreciated. ------=_NextPart_000_01BC28C2.09CFA1A0 Content-Type: application/octet-stream; name="cryojour4.txt" Content-Transfer-Encoding: quoted-printable Content-Description: cryojour4 (Text Document) Content-Disposition: attachment; filename="cryojour4.txt" <!DOCTYPE htmlplus PUBLIC -//Internet/RFC xxxx//EN> <HTMLPLUS> <HEAD><TITLE>Critical Cooling and Warming Rates to Avoid Ice = Crystallization in Small Pieces of Mammalian Organs Permeated with = Cryoprotective Agents</TITLE></HEAD> <BODY BGCOLOR=3D"ffffff"> <BANNER> <!-- Toolbar --> <A = HREF=3D"/cgi-bin/delta.bids.ac.uk_8006/class;CFmedia::CMDApplication;home= /0104040a010802560f04020204560100050b000106000b55530352535004060c05570003= 5950510f56530700035c520350505b570304550c0250560d52570203595701000003/130:= 1124:17745"><IMG SRC=3D"/gui/bitmaps/menu.gif" BORDER=3D"0" = ALT=3D"Menu"></A> <A = HREF=3D"/cgi-bin/delta.bids.ac.uk_8006/display/0104040a010802520f07070506= 5203030f000002040e035654025755510d0707065608055151500951560c0202545308505= 65150050051030252540759540207555705070e07/130:1120:2206"><IMG = SRC=3D"/gui/bitmaps/up.gif" BORDER=3D"0" ALT=3D"Up"></A> <A = HREF=3D"/cgi-bin/delta.bids.ac.uk_8006/fetch/0104040a010802520f0707050652= 03030f000002040e035654025755510d0707065608055151500951560c020254530850565= 150050051030252540759540207555705070e07/130:1120:2206/5"><IMG = SRC=3D"/gui/bitmaps/previous.gif" BORDER=3D"0" ALT=3D"Previous"></A> <A = HREF=3D"/cgi-bin/delta.bids.ac.uk_8006/fetch/0104040a010802520f0707050652= 03030f000002040e035654025755510d0707065608055151500951560c020254530850565= 150050051030252540759540207555705070e07/130:1120:2206/7"><IMG = SRC=3D"/gui/bitmaps/next.gif" BORDER=3D"0" ALT=3D"Next"></A> <A = HREF=3D"/cgi-bin/delta.bids.ac.uk_8006/showfind/0104040a010802560f0402020= 4560100050b000106000b55530352535004060c055700035950510f56530700035c520350= 505b570304550c0250560d52570203595701000003/130:1124:17745/0"><IMG = SRC=3D"/gui/bitmaps/search.gif" BORDER=3D"0" ALT=3D"Search"></A> <A = HREF=3D"/cgi-bin/delta.bids.ac.uk_8006/display/0104040a0108015a0f04050708= 5203030f0000010c0e0054560c5755510d0707055e080653535e0951560c0202575b08535= 4535e050051030252570f595700055b5705070e07/130:1118:1028"><IMG = SRC=3D"/gui/bitmaps/services2.gif" BORDER=3D"0" ALT=3D"Services"></A> <A HREF=3D/help/help9.html><IMG SRC=3D"/gui/bitmaps/help.gif" = BORDER=3D"0" ALT=3D"HelpArticle"></A> </BANNER> <IMG SRC =3D"/gui/bitmaps/smallideal.gif" ALT=3D"ideal"><BR> <IMG SRC=3D"/gui/bitmaps/separator.gif" ALT=3D"separator"> <BR> <TABLE ALIGN=3DLEFT WIDTH=3D70%> <TR> <TD VALIGN=3DTOP> <A HREF=3D"http://www.apnet.com/www/journal/cy.htm"><IMG SRC=3D" /gui/logos/cy.gif" ALIGN=3D"LEFT" ALT=3D"Logo"></A><BR CLEAR=3DALL> <FONT SIZE=3D-1><B>Click journal logo for more = information</B></FONT><BR> </TD> <TD VALIGN=3DTOP> </TD></TR></TABLE> <BR CLEAR=3DALL> <H3>Critical Cooling and Warming Rates to Avoid Ice Crystallization in = Small Pieces of Mammalian Organs Permeated with Cryoprotective = Agents</H3> J. F. Peyridieu, A. Baudot, P. Boutron, J. Mazuer, J. Odin, A. Ray, = E. Chapelier, E. Payen, J. L. Descotes<P> <P>Measurements were made by = differential scanning calorimetry on small pieces of rabbit kidney = permeated with 2,3-butanediol containing mainly the levo- and = dextro-isomers. The critical cooling rates necessary to vitrify the = pieces of organ, and the corresponding critical warming rates which are = required to avoid crystallization in the vitrified samples, were = determined. The dynamic method used for these determinations is = described. The glass-forming tendency and the stability of the amorphous = state were both greater in the kidney tissue samples than in the bulk = cryoprotective solution. This result is discussed in the context of the = lowering of the freezing point of water in emulsions and the promotion = of supercooling in hydrogels and porous materials. In corresponding = experiments with rat hearts impregnated with 1,2-propanediol, only the = critical warming rate was reduced.</P> <P><I>Cryobiology</I>, v 33, n 4, = August 1996, p436-446 (ID CY960044) <BR><I>Copyright © 1996 Academic Press, Inc. </I><BR><BR = CLEAR=3DALL><IMG SRC=3D"/gui/bitmaps/separator.gif" ALIGN=3DCENTER><P><A = NAME=3D"130:1042:1661"></A><A HREF=3D"/index.html"><IMG = SRC=3D"/gui/bitmaps/home.gif" ALT=3D"Home" ALIGN=3DLEFT BORDER=3D0><A = NAME=3D"130:1042:1662"></A></A><A HREF=3D"/related.html"><IMG = SRC=3D"/gui/bitmaps/related.gif" ALT=3D"Related sites" BORDER=3D0></A><A = NAME=3D"130:1042:1663"></A></BODY></HTMLPLUS> ------=_NextPart_000_01BC28C2.09CFA1A0-- Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=7807