X-Message-Number: 8075 Date: Mon, 14 Apr 1997 16:36:58 -0700 (PDT) From: Olaf Henny <> Subject: CRYONICS Re: CryoNet #8060 - #8068 >>I wrote the following in an earlier message and I am sorry to say, that I did not receive a reply from Prof. Peter Sharp or anybody else from the Roslin Institute: >""""""""""""""""""""""""""""""""""""""""""""""""""""" >In Message #7930 Robert Ettinger wrote: > >>3. Olaf Henny (#7913) makes me wonder if I bungled in skimming the news on >>Dolly. Olaf says the offspring was from an egg (sheep B), tickled by an adult >>udder cell nucleus from sheep A, then gestated in sheep C, and that the DNA >>in the udder cell was not expressed or passed on. Am I confused? If the above >>is correct, it would not be cloning at all--just another slight variation of >>parthenogenesis. My understanding was that the udder cell nucleus was the >>sole provider of the genetic material of the offspring--that's what made it >>cloning, use of an adult somatic cell to reproduce its own genotype. > >In Message 7913 I summarized my understanding of the procedure used in cloning as follows: >----------------------------------------------------------- >The no doubt significant accomplishment of Ian Wilmut and >Keith Campbell was, that they managed to 'bully' through >starvation an egg of sheep A into accepting the cell of an >udder of sheep B in lieu of a sperm. The resulting zygote >was then implanted into the uterus of sheep C and brought to >term in the usual manner of natural propagation. The >accomplishment of these Scottish scientists lay in the >acceptance of the udder cell in lieu of sperm and complete >dominance of the DNA of the egg, which was presumably due to >the inability of a somatic cell to assert its own DNA. >------------------------------------------------------------ >This was based on press reports I have read, which are not >necessarily reliable. I have therefore after Robert Ettinger's >questioning endeavored to search the net for more pertinent >information and quickly got mired in a proliferation of >sensationalized press reports. I have therefore sent an e-mail >to Dr. Ian Wilmut requesting an abstract or brief summary of the >steps taken in the successful cloning through the following website: > >Roslin Institute > Current projects: January 1997 > >Programme 03: >Developmental and reproductive biology of farm animals >Programme Coordinator: Prof. Peter Sharp > ><http://www.ri.bbsrc.ac.uk/library/projects/prog03.html> > >If and when I receive a reply, I will definitely post it here. > >Olaf Henny """"""""""""""""""""""""""""""""""""""""""""""""""""""""""""" Olaf Henny Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=8075