X-Message-Number: 8427
Date: Wed, 30 Jul 1997 09:30:25 -0700 (PDT)
From: Doug Skrecky <>
Subject: cooking with adonitol

Adonitol has been used to advantage in cryopreservation. Water is added to
plasticize it so that cooking it is not required to liquify it. :)

Cryoprotectant    Percentage Survival of Frozen/Thawed Rat Embryos
                     0.3 M      1.0 M
DMSO                 20%        60%
glycerol             16         52
adonitol             67         88
sorbitol             58         60

Reference:
"Cryoprotective Effect of Polyols on Rat Embryos During Two-Step Freezing"
Cryobiology 29: 332-341 1992

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