X-Message-Number: 8597
Date: Mon, 15 Sep 1997 18:49:38 -0700 (PDT)
From: Doug Skrecky <>
Subject: 16'th update on fly longevity experiments 

    This is the sixteenth update of my fly experiments. The heat wave is
 over so I expect life spans will be greater in the sixth run than in the
 fifth run. In the fifth run all that looks interesting is activated
 charcoal, sucrose and l-proline. Since activated charcoal significantly
 outperformed controls in the 3'rd run, I have shortlisted this as a
 "probable" life span prolonging supplement for flies.
    Since the lower dosage of a supplement almost always results in a
 greater life span, I am decreasing the dosages I use in the sixth run. I
 am retesting l-proline as well as sage and paprika here. For the first
 time I am boiling the water I use. Although flies live longer when live
 yeast is added to their food, this result was obtained under highly
 sterile laboratory conditions. This might not apply to my less than
 completely sanitary home experiments. So I am running two control
 bottles, one with added yeast from each breeding bottle to test for the
 effect of live yeast. None of the supplement bottles contains live yeast
 in this run. The breeding bottles were all created 20 days ago, using
 boiled water and a high dose (1.04 gm) of activated charcoal added to 20
 gm 4-24 medium.

 Fifth Run                  Survival
 BB#   Supplement           DAY 16 DAY 23 DAY 26 DAY 30 DAY 36
  1    control#1             10%    10%     0%     0%   all 0%
  1    l-alanine             13      0      0      0
  1    l-alanine 4X           0      0      0      0
  1    anise                 10      0      0      0
  1    anise 4X               0      0      0      0
  1    l-glutamine           13      0      0      0
  1    l-glutamine 4X         0      0      0      0
  1    malt                  25      0      0      0
  1    malt 4X                9      0      0      0
  1    paba                  15      0      0      0
  1    paba 4X                0      0      0      0

  2    control#2             22     13      4      0
  2    l-cysteine            18      0      0      0
  2    l-cysteine 4X          0      0      0      0
  2    l-glycine             10      0      0      0
  2    l-glycine 4X           0      0      0      0
  2    l-isoleucine          11      0      0      0
  2    l-isoleucine 4X        3      0      0      0
  2    l-leucine             11      0      0      0
  2    dl-phenylalanine       8      0      0      0
  2    dl-phenylalanine 4X   17      0      0      0
  2    l-proline             27     13      7      7
  2    l-proline 4X          26      5      0      0
  2    l-threonine           22      6      6      0
  2    l-threonine 4X         0      0      0      0
  2    l-valine              13      4      0      0
  2    l-valine 4X           17      0      0      0

  3    activated charcoal    27     13      0      0
  3    activated charcoal 4X 40     20     20      0
  3    glucose               24      8      8      0
  3    glucose 4X            28      9      0      0
  3    l-leucine 4X           6      0      0      0
  3    sorbitol              25      8      4      0
  3    sorbitol 4X           19      6      0      0
  3    sucrose               32     21     11      0
  3    sucrose 4X            15      0      0      0

  4    control#4             65     44     20      8
  4    l-carnosine           39     25     13      4
  4    l-carnosine 2X        55     27     12      0
  4    l-carnosine 4X        33      7      3      3
  4    l-carnosine 8X        15      3      3      0

 Sixth Run
 BB# Supplement   Amount
  1  cntl 1
  1  cntl 1 +yeast
  1  B complex: 1/4 capsule
  1  B complex 4X: 1 capsule: (50 mg B1, 50 mg B2, 50 mg B3,
                   50 mg B5, 50 mg B6, 50 mcg B12, 60 mcg biotin,
                   1 mg folic acid, 50 mg choline bitartrate,
                   50 mg inositol, 50 mg PABA)
  1  chitosan: 1/4 capsule 75 mg
  1  chitosan 4X: 1 capsule 300 mg
  1  chondroitin sulfate: 1/4 capsule 100 mg
  1  chondroitin sulfate 4X: 1 capsule 400 mg
  1  chromium picolinate: 1/4 capsule 125 mcg
  1  chromium picolinate 4X: 1 capsule 500 mcg
  1  cinnamon: 1/16 teaspoon
  1  cinnamon 4X: 1/4 teaspoon
  1  DMAE bitartrate: 1/4 capsule 37.5 mg
  1  DMAE bitartrate 4X: 1 capsule 150 mg

  2  cntl 2
  2  cntl 2 +yeast
  2  french mushroom: 1/16 teaspoon
  2  french mushroom 4X: 1/4 teaspoon
  2  kava kava: 1/4 capsule 11.25 mg kavalactones
  2  kava kava 4X: 1 capsule 45 mg kavalactones
  2  onion: 1/16 teaspoon
  2  onion 4X: 1/4 teaspoon
  2  paprika, spanish: 1/16 teaspoon
  2  paprika, spanish 4X: 1/4 teaspoon
  2  l-proline: 1/4 capsule 125 mg
  2  l-proline 4X: 1 capsule 500 mg
  2  sage: 1/16 teaspoon
  2  sage 4X: 1/4 teaspoon
  2  sodium citrate: 1/16 teaspoon 375 mg
  2  sodium citrate 4X: 1/4 teaspoon 1500 mg

  3  cntl 3
  3  cntl 3 +yeast
  3  St John's Wort: 1/4 capsule 225 mcg hypericin
  3  St John's Wort 4X: 1 capsule 900 mcg hypericin
  3  sumac: 1/16 teaspoon
  3  sumac 4X: 1/4 teaspoon
  3  wild yam: 1/4 capsule 125 mg
  3  wild yam 4X: 1 capsule 500 mg

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