X-Message-Number: 9532
Date: Tue, 21 Apr 1998 11:02:13 -0700 (PDT)
From: Doug Skrecky <>
Subject: cryopreservation of kidney

Authors
  Fahy GM.  Ali SE.
Institution
  Transfusion and Cryopreservation Research Program, Naval Medical Research
  Institute, Building 29, 8901 Wisconsin Avenue, Bethesda, Maryland 20889, USA.
Title
  Cryopreservation of the mammalian kidney. II. Demonstration of immediate ex
  vivo function after introduction and removal of 7.5 M cryoprotectant.
Source
  Cryobiology.  35(2):114-31, 1997 Sep.
Abstract
  The objective of the present study was to determine whether rabbit kidneys
  could be perfused with a 7.5 M vitrification solution (VS4, which vitrifies
  under applied pressure) without loss of function. To answer this question,
  kidneys were perfused with VS4 using a computer-based machine to gradually
  raise and lower concentration and then attached to the aorta and vena cava of
  a perfusor rabbit using an apparatus that permitted renal blood flow and
  renal function to be measured. About half (6/13) of the kidneys so evaluated
  resumed substantial immediate function after a transient period of severely
  reduced blood flow. Loss of function did not occur if cryoprotectant
  concentration was limited to 3.8 M. The loss of function produced by VS4
  could be partially reproduced by artificially limiting blood reflow in
  control kidneys to simulate the transiently low flows caused by VS4 exposure.
  These results provide the first evidence that both the parenchyma and the
  vascular system of a sensitive mammalian organ can survive exposure to a
  vitrifiable concentration of cryoprotectant. Copyright 1997 Academic Press.

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