X-Message-Number: 9544
Date: Sat, 25 Apr 1998 05:09:58 -0700 (PDT)
From: Doug Skrecky <>
Subject: ethylene glycol and DMSO

Authors
  Sakonju I.  Taura Y.  Inayoshi Y.  Suzuki T.  Takimoto K.  Nakaichi M. 
  Nakama S.
Institution
  Department of Veterinary Surgery, Yamaguchi University, Japan.
Title
  Cryopreservation of isolated rat islets of Langerhans in the presence of
  ethylene glycol or dimethyl sulfoxide: evaluation of toxicity and the dynamic
  pattern of subsequent insulin release in vitro.
Source
  Cryobiology.  33(3):354-62, 1996 Jun.
Abstract
  The toxic effect of ethylene glycol (EG) on the pattern of dynamic insulin
  release from rat pancreatic islets with or without freezing was investigated
  in comparison with that of dimethyl sulfoxide (Me2SO). Sixty islets were
  perifused (1 ml/min) consecutively with D-glucose (1.67 mM for 30 min
  followed by 16.7 mM for 60 min and 1.67 mM for 60 min) after exposure to 2.0
  M EG or Me2SO for 1 h at either 22 or 0 degrees C. During the second period
  of perifusion, the insulin output from islets exposed to Me2SO or EG at 22
  degrees C decreased to 53 and 51% of that from nontreated control islets,
  respectively. On the other hand, the islets exposed to EG at 0 degrees C
  exhibited 86% of the control insulin output under the same perifusion
  conditions, and this appeared to be higher than that of islets exposed to
  Me2SO (60%) at 0 degrees C. Frozen islets, after exposure to 2.0 M EG or
  Me2SO for 1 h at 0 degrees C, responded positively to 16.7 mM D-glucose, and
  the typical biphasic pattern of insulin secretion was observed. The insulin
  output from these islets during the second period of perifusion was not
  comparable to that from unfrozen control islets. In particular, the mean
  insulin output of EG-cryopreserved islets during the second period accounted
  for 99% of that from unfrozen control islets. The present findings suggest
  the possible use of EG as an alternative cryoprotectant to Me2SO.

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