X-Message-Number: 9553
Date: Mon, 27 Apr 1998 13:19:00 -0700 (PDT)
From: Doug Skrecky <>
Subject: working with slices

Authors
  Fisher RL.  Hasal SJ.  Sanuik JT.  Hasal KS.  Gandolfi AJ.  Brendel K.
Institution
  Department of Pharmacology, University of Arizona, Tucson, Arizona, 85724,
  USA.
Title
  Cold- and cryopreservation of dog liver and kidney slices.
Source
  Cryobiology.  33(1):163-71, 1996 Feb.
Abstract
  The use of tissue slices in culture could decrease the number of animals used
  in health-related research and decrease experimental variation. This
  reduction may come about particularly if the methods of cold- and
  cryopreserving tissue slices are perfected, and one can conduct sequential in
  vitro experiments into xenobiotic metabolism, organ-specific toxicity, or
  organ-specific biochemical processes with tissue slices. With this goal in
  mind, dog liver and kidney slices were placed in cold storage at 0 degrees C
  using Viaspan (UW), Euro-Collins (EC), Sacks + prostacyclin (SP), and V-7
  (V7) cold-preservation solutions for 10 days. Viability was assessed each day
  by measuring K+ content and protein synthesis after 4 h of incubation in
  Waymouth + 10% fetal calf serum (FCS). Dog liver slices can be cold-preserved
  in V7 for up to 7 days using K+ retention as the viability criterion but only
  up to 4 days using protein synthesis. Dog kidney slices can be cold-preserved
  in UW, EC, and V7 for up to 10 days using K+ retention, but only V7 could
  maintain protein synthesis for 10 days. Cryopreserved dog liver and kidney
  slices retained 63-68% of control viability after 4 h of incubation in FCS.
  The cryopreservation regimen included using 10% dimethyl sulfoxide in FCS as
  the cryoprotectant, a freezing rate of 0.5 degrees C/min for liver slices and
  12 degrees C/min for kidney slices, and thawing in 37 degrees C FCS.
  Continued development of cold- and cryopreserving tissue slices could reduce
  the numbers of animals used and provide accurate and reproducible data.

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