X-Message-Number: 9667 From: Olaf Henny <> Subject: Message #9655 From Charles Platt: Right Date: Sat, 9 May 1998 12:47:39 -0700 To: Message #9655 From Charles Platt: Right, It has all been said before and clearly will have have to be said again and again and again and obviously not just to newcomers of cryonics. TO General: I am attaching below a copy of an abstract, which I ran into on Medline. I think I have heard somewhere, that the liver is the organ most similar to the brain in tissue structure, so there may be some useful info. contained therein. Best, Olaf Biochemistry (Mosc) 1998 Feb;63(2):219-223 An Isocratic, Reversed-Phase HPLC Method for the Determination of Postischemic Efflux of Purines and Pyrimidines during Reperfusion of Isolated Liver. Toshchakov VY, Bashkina LV, Onishchenko NA, Shumakov VI Institute of Transplantation and Artificial Organs, Ministry of Health Care of the Russian Federation, ul. Shchukinskaya 1, Moscow, 123182 Russia. [Record supplied by publisher] An isocratic, reversed-phase HPLC method was developed for the determination of the rates of purine and pyrimidine efflux during early reperfusion of isolated organs after non-perfusion cold conservation. The method enables determination of uric acid, cytidine, xanthine, hypoxanthine, uridine, AMP, inosine, and adenosine in liver perfusate using a standard C-18 column (25 cm length). Peaks are resolved by elution with buffer containing 1% acetonitrile, 20 mM potassium citrate (pH 6.25), and 25-55 mM tetramethylammonium. The effects of pH and solvents on peak retention times are described. As an example of the application of the method, the effects of allopurinol on the rates of postischemic efflux of purines and pyrimidines during reperfusion of liver stored in the cold for 24 h in Euro-Collins solution was studied. PMID: 9526118 Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=9667