X-Message-Number: 9667
From: Olaf Henny <>
Subject: Message #9655 From Charles Platt: Right
Date: Sat, 9 May 1998 12:47:39 -0700

To: Message #9655 From Charles Platt:
Right, It has all been said before and clearly will have have to 
be said again and again and again and obviously not just to 
newcomers of cryonics.

TO General:
I am attaching below a copy of an abstract, which I ran into on 
Medline.  I think I have heard somewhere, that the liver is the 
organ most similar to the brain in tissue structure, so there 
may be some useful info. contained therein.

Best,

Olaf

Biochemistry (Mosc) 1998 Feb;63(2):219-223 

An Isocratic, Reversed-Phase HPLC Method for the Determination of
Postischemic Efflux of Purines and Pyrimidines during Reperfusion 
of Isolated Liver.

Toshchakov VY, Bashkina LV, Onishchenko NA, Shumakov VI

Institute of Transplantation and Artificial Organs, Ministry of 
Health Care of the Russian Federation, ul. Shchukinskaya 1, 
Moscow, 123182 Russia. 

[Record supplied by publisher]

An isocratic, reversed-phase HPLC method was developed for the 
determination of the rates of purine and pyrimidine efflux
during early reperfusion of isolated organs after non-perfusion 
cold conservation. The method enables determination of uric
acid, cytidine, xanthine, hypoxanthine, uridine, AMP, inosine, 
and adenosine in liver perfusate using a standard C-18 column
(25 cm length). Peaks are resolved by elution with buffer 
containing 1% acetonitrile, 20 mM potassium citrate (pH 6.25), 
and 25-55 mM tetramethylammonium. The effects of pH and solvents 
on peak retention times are described. As an example of the
application of the method, the effects of allopurinol on the 
rates of postischemic efflux of purines and pyrimidines during
reperfusion of liver stored in the cold for 24 h in Euro-Collins 
solution was studied. 

PMID: 9526118

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