X-Message-Number: 9737
Date: Mon, 18 May 1998 21:18:47 -0700 (PDT)
From: Doug Skrecky <>
Subject: dried red blood cells

Authors
  Sowemimo-Coker SO.  Goodrich RP.  Zerez CR.  Tanaka KR.
Institution
  Department of Basic Blood Research, Cryopharm Corporation, Pasadena,
  California.
Title
  Refrigerated storage of lyophilized and rehydrated, lyophilized human red
  cells.
Source
  Transfusion.  33(4):322-9, 1993 Apr.
Abstract
  Human red cells (RBCs) were collected in CPDA-1 and then
  freeze-dried in lyoprotective solution. The lyophilized RBCs
  were then stored at -20 degrees C for 7 days. At the end of the storage
  period, the lyophilized RBCs were rehydrated and washed in dextrose saline.
  The washed, reconstituted, lyophilized RBCs were resuspended in final wash
  solutions of ADSOL, CPDA-1, or a special additive solution containing
  glucose, citrate, phosphate, adenine, and mannitol, and then
  they were stored at 4 degrees C for an additional 7 days. The main purpose of
  this study was to determine whether human RBCs can be lyophilized in such a
  manner that normal metabolic, rheologic, and cellular properties are
  maintained during rehydration and subsequent storage in standard blood bank
  preservative solutions. Our results show that reconstituted, lyophilized RBCs
  maintained levels of ATP, 2,3 DPG, lactate, and cellular properties that are
  equal to or better than those in control nonlyophilized RBCs stored for a
  comparable period in CPDA-1. Reconstituted, lyophilized RBCs stored at 4
  degrees C after rehydration also show better maintenance of ATP, 2,3 DPG, and
  lactate than do control RBCs stored in the same preservative solutions for
  comparable periods.

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