X-Message-Number: 9831 Date: Mon, 01 Jun 1998 03:49:45 -0700 From: Paul Wakfer <> Subject: Re: CryoNet #9826 Lost Brain Information References: <> > Message #9826 > Date: Sun, 31 May 1998 12:43:08 -0700 (PDT) > From: John K Clark <> > Subject: Lost Brain Information > In #9814 On Sat, 30 May 1998 Paul Wakfer <> Wrote: > > >the thawing process had partially masked the damage. When brain > >slices were "freeze substituted" (ie. the ice remove in situ) and > >then fixed, the true nature and extent of the damage was seen. > > > I don't understand, it seems to me the thawing process couldn't possibly do > anything except increase the amount of damage. While you are quite correct that the damage will *be* greater after thawing, instead of being shrunken and separated by ice holes strewn with debris, the tissue has now swollen and rejoined its neighbors so that the damage of holes and debris is less visible. Again, I am no expert here, but this is my understanding. > >How about making a tax deductible donation to the Institute for > >Neural Cryobiology's Hippocampal Slice Vitrification Project? > > > OK, that's a reasonable criticism, I guess it's time for me to put some money > were my mouth is. Could you remind me how to write out the check and where to > send it? Thanks for rising to the challenge :-) The project is slowly making its way through the approval process at the university medical center research institute. It has received formal approval of the institution's Animal Care and Use Review Committee. The committee which assesses the sufficiency of the "worth" of the research is waiting on a slow reviewer to report back to them. Then, I expect the contracts office will probably want some changes to the draft cost and benefit sharing agreement which I have sent them. Any funding that anyone wishes to send at this time it welcome, but I am waiting for the project approval before I make a special appeal which will say "now is the time to make good on your support for brain cryopreservation research". However, it is always better to have the money ahead of time, since then I can move more quickly to start the project once it is approved. Please make out and send your check to: The Institute for Neural Cryobiology 8439 White Oak Ave #110 Rancho Cucamonga, CA 91730-3860 -- Paul -- Voice/Fax: 909-481-9620 Page: 800-805-2870 The Institute for Neural Cryobiology - http://neurocryo.org Perfected cryopreservation of Central Nervous System tissue for neuroscience research and medical repair of brain diseases Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=9831